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immunofluorescence microscopy  (Olympus)


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    Olympus immunofluorescence microscopy
    Immunofluorescence Microscopy, supplied by Olympus, used in various techniques. Bioz Stars score: 99/100, based on 24421 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/immunofluorescence+microscopy/FV3000+Confocal+Laser+Scanning+Microscope/us12460007-1561-0-5
    Average 99 stars, based on 24421 article reviews
    immunofluorescence microscopy - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: Antimicrobial Effects of Thonningianin a (TA)-Loaded Chitosan Nanoparticles Encapsulated by a PF-127 hydrogel in Diabetic Wound Healing
    Article Snippet: .. The cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 5% BSA, incubated overnight with primary antibodies at 4 °C, incubated with secondary antibodies at room temperature, and subjected to DAPI staining, after which photographs were taken via immunofluorescence microscopy (Olympus BX53, Japan). https://doi.org/10.2147/IJN.S488115 DovePress International Journal of Nanomedicine 2024:19 12840 Powered by TCPDF (www.tcpdf.org) Flow Cytometry After RAW264.7 cells were seeded on plates, the control group, diabetes group, diabetes + TA group and TA group were established. ..

    Article Title: Antimicrobial Effects of Thonningianin a (TA)-Loaded Chitosan Nanoparticles Encapsulated by a PF-127 hydrogel in Diabetic Wound Healing
    Article Snippet: .. The cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 5% BSA, incubated overnight with primary antibodies at 4 °C, incubated with secondary antibodies at room temperature, and subjected to DAPI staining, after which photographs were taken via immunofluorescence microscopy (Olympus BX53, Japan). ..

    Staining:

    Article Title: Antimicrobial Effects of Thonningianin a (TA)-Loaded Chitosan Nanoparticles Encapsulated by a PF-127 hydrogel in Diabetic Wound Healing
    Article Snippet: .. The cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 5% BSA, incubated overnight with primary antibodies at 4 °C, incubated with secondary antibodies at room temperature, and subjected to DAPI staining, after which photographs were taken via immunofluorescence microscopy (Olympus BX53, Japan). https://doi.org/10.2147/IJN.S488115 DovePress International Journal of Nanomedicine 2024:19 12840 Powered by TCPDF (www.tcpdf.org) Flow Cytometry After RAW264.7 cells were seeded on plates, the control group, diabetes group, diabetes + TA group and TA group were established. ..

    Article Title: Antimicrobial Effects of Thonningianin a (TA)-Loaded Chitosan Nanoparticles Encapsulated by a PF-127 hydrogel in Diabetic Wound Healing
    Article Snippet: .. The cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 5% BSA, incubated overnight with primary antibodies at 4 °C, incubated with secondary antibodies at room temperature, and subjected to DAPI staining, after which photographs were taken via immunofluorescence microscopy (Olympus BX53, Japan). ..

    Immunofluorescence:

    Article Title: Antimicrobial Effects of Thonningianin a (TA)-Loaded Chitosan Nanoparticles Encapsulated by a PF-127 hydrogel in Diabetic Wound Healing
    Article Snippet: .. The cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 5% BSA, incubated overnight with primary antibodies at 4 °C, incubated with secondary antibodies at room temperature, and subjected to DAPI staining, after which photographs were taken via immunofluorescence microscopy (Olympus BX53, Japan). https://doi.org/10.2147/IJN.S488115 DovePress International Journal of Nanomedicine 2024:19 12840 Powered by TCPDF (www.tcpdf.org) Flow Cytometry After RAW264.7 cells were seeded on plates, the control group, diabetes group, diabetes + TA group and TA group were established. ..

    Article Title: Targeting LLT1 as a potential immunotherapy option for cancer patients non-responsive to existing checkpoint therapies in multiple solid tumors
    Article Snippet: .. Immunofluorescence microscopy was performed on the Olympus FV3000- 4 laser scanning confocal microscope with a 60X magnification, 1.35-NA objective. ..

    Article Title: Vagal sensory neuron-derived FGF3 controls insulin secretion.
    Article Snippet: Short article Vagal sensory neuron-derived FGF3 controls insulin secretion

    Article Title: Antibodies that bind to c-type lectin domain family 2 member d (CLEC2D)
    Article Snippet: .. Immunofluorescence microscopy was performed on Olympus FV3000-4 laser scanning confocal microscope with a 60× magnification with 1.35-NA objective. ..

    Article Title: Antibodies that bind to C-type lectin domain family 2 member D (CLEC2D)
    Article Snippet: .. Immunofluorescence microscopy was performed on Olympus FV3000-4 laser scanning confocal microscope with a 60× magnification with 1.35-NA objective. ..

    Article Title: Antimicrobial Effects of Thonningianin a (TA)-Loaded Chitosan Nanoparticles Encapsulated by a PF-127 hydrogel in Diabetic Wound Healing
    Article Snippet: .. The cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 5% BSA, incubated overnight with primary antibodies at 4 °C, incubated with secondary antibodies at room temperature, and subjected to DAPI staining, after which photographs were taken via immunofluorescence microscopy (Olympus BX53, Japan). ..

    Article Title: Caffeic acid and diabetic neuropathy: Investigating protective effects and insulin-like growth factor 1 (IGF-1)-related antioxidative and anti-inflammatory mechanisms in mice
    Article Snippet: Subsequently, these sections were washed with PBS and further incubated with Cy3-labeled goat anti-rabbit (1:400; Abcam, #ab6939), DyLight® 488-labeled goat anti-rabbit (1:400; Abcam, #ab96899), or DyLight® 488-labeled goat anti-mouse secondary antibodies (1:400; Abcam, #ab98794) for 2 h at ambient temperature, with the choice of secondary antibody being contingent upon the species origin of the primary antibody. .. Image capturing was conducted using immunofluorescence microscopy (Olympus BX53; Olympus, Tokyo, Japan) at magnifications of 200 × or 400 × . ..

    Microscopy:

    Article Title: Antimicrobial Effects of Thonningianin a (TA)-Loaded Chitosan Nanoparticles Encapsulated by a PF-127 hydrogel in Diabetic Wound Healing
    Article Snippet: .. The cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 5% BSA, incubated overnight with primary antibodies at 4 °C, incubated with secondary antibodies at room temperature, and subjected to DAPI staining, after which photographs were taken via immunofluorescence microscopy (Olympus BX53, Japan). https://doi.org/10.2147/IJN.S488115 DovePress International Journal of Nanomedicine 2024:19 12840 Powered by TCPDF (www.tcpdf.org) Flow Cytometry After RAW264.7 cells were seeded on plates, the control group, diabetes group, diabetes + TA group and TA group were established. ..

    Article Title: Targeting LLT1 as a potential immunotherapy option for cancer patients non-responsive to existing checkpoint therapies in multiple solid tumors
    Article Snippet: .. Immunofluorescence microscopy was performed on the Olympus FV3000- 4 laser scanning confocal microscope with a 60X magnification, 1.35-NA objective. ..

    Article Title: Vagal sensory neuron-derived FGF3 controls insulin secretion.
    Article Snippet: Short article Vagal sensory neuron-derived FGF3 controls insulin secretion

    Article Title: Antibodies that bind to c-type lectin domain family 2 member d (CLEC2D)
    Article Snippet: .. Immunofluorescence microscopy was performed on Olympus FV3000-4 laser scanning confocal microscope with a 60× magnification with 1.35-NA objective. ..

    Article Title: Antibodies that bind to C-type lectin domain family 2 member D (CLEC2D)
    Article Snippet: .. Immunofluorescence microscopy was performed on Olympus FV3000-4 laser scanning confocal microscope with a 60× magnification with 1.35-NA objective. ..

    Article Title: Antimicrobial Effects of Thonningianin a (TA)-Loaded Chitosan Nanoparticles Encapsulated by a PF-127 hydrogel in Diabetic Wound Healing
    Article Snippet: .. The cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 5% BSA, incubated overnight with primary antibodies at 4 °C, incubated with secondary antibodies at room temperature, and subjected to DAPI staining, after which photographs were taken via immunofluorescence microscopy (Olympus BX53, Japan). ..

    Article Title: Caffeic acid and diabetic neuropathy: Investigating protective effects and insulin-like growth factor 1 (IGF-1)-related antioxidative and anti-inflammatory mechanisms in mice
    Article Snippet: Subsequently, these sections were washed with PBS and further incubated with Cy3-labeled goat anti-rabbit (1:400; Abcam, #ab6939), DyLight® 488-labeled goat anti-rabbit (1:400; Abcam, #ab96899), or DyLight® 488-labeled goat anti-mouse secondary antibodies (1:400; Abcam, #ab98794) for 2 h at ambient temperature, with the choice of secondary antibody being contingent upon the species origin of the primary antibody. .. Image capturing was conducted using immunofluorescence microscopy (Olympus BX53; Olympus, Tokyo, Japan) at magnifications of 200 × or 400 × . ..

    Flow Cytometry:

    Article Title: Antimicrobial Effects of Thonningianin a (TA)-Loaded Chitosan Nanoparticles Encapsulated by a PF-127 hydrogel in Diabetic Wound Healing
    Article Snippet: .. The cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 5% BSA, incubated overnight with primary antibodies at 4 °C, incubated with secondary antibodies at room temperature, and subjected to DAPI staining, after which photographs were taken via immunofluorescence microscopy (Olympus BX53, Japan). https://doi.org/10.2147/IJN.S488115 DovePress International Journal of Nanomedicine 2024:19 12840 Powered by TCPDF (www.tcpdf.org) Flow Cytometry After RAW264.7 cells were seeded on plates, the control group, diabetes group, diabetes + TA group and TA group were established. ..

    Control:

    Article Title: Antimicrobial Effects of Thonningianin a (TA)-Loaded Chitosan Nanoparticles Encapsulated by a PF-127 hydrogel in Diabetic Wound Healing
    Article Snippet: .. The cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 5% BSA, incubated overnight with primary antibodies at 4 °C, incubated with secondary antibodies at room temperature, and subjected to DAPI staining, after which photographs were taken via immunofluorescence microscopy (Olympus BX53, Japan). https://doi.org/10.2147/IJN.S488115 DovePress International Journal of Nanomedicine 2024:19 12840 Powered by TCPDF (www.tcpdf.org) Flow Cytometry After RAW264.7 cells were seeded on plates, the control group, diabetes group, diabetes + TA group and TA group were established. ..



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    Cell Signaling Technology Inc immunofluorescence if microscopy
    ( A ) Left : Protein–protein interaction network centered on P4HA2, illustrating its context-dependent interactions with ER- and nucleoplasm-localized proteins. Right : Real P4HA2 images (red border) and ProtiCelli -generated images of ten interaction partners for two representative cells, grouped by interaction context (gold: ER/collagen; blue: mRNA processing). The rightmost column shows the binarized spatial interaction overlap between P4HA2 and the mean projection of proteins within partner groups. Scale bar, 10 µm. ( B ) Representative real and ProtiCelli -generated fluorescence <t>microscopy</t> images for four proteins (MECP2, BABAM2, YWHAB, BPTF) under Paclitaxel, untreated, and Vorinostat conditions, demonstrating the model’s ability to capture single-cell expression variance across treatment contexts. Scale bar, 10 µm. ( C ) Boxplots comparing ProtiCelli -generated versus real normalized protein intensities for ten representative proteins across treatment conditions. Median values are annotated; statistical significance (Mann-Whitney U test) is indicated by bracket annotations. ( D ) Scatter plots comparing average foreground intensity between real and ProtiCelli -generated images for twelve cell cycle-regulated proteins. Each point represents one single cell and the red line denotes the linear fit with 95% confidence interval. Pearson correlation coefficients ( r ) are reported per protein. ( E ) Representative real and ProtiCelli -generated <t>immunofluorescence</t> images of the FUCCI markers CDT1 (A-549) and GMNN (MDA-MB-467). Protein signal is shown in green; microtubule staining (red), derived from real images, is identical across all panels. Scale bar, 10 µm. ( F, G ) Generated versus measured FUCCI marker intensities of CDT1 and Geminin (GMNN) across cell cycle stages (G1, G1/S, G2) without fine-tuning, after fine-tuning, and for real images, in A-549 ( F ) and MDA-MB-468 ( G ) cell lines. Fine-tuned ProtiCelli accurately capture cell-cycle-dependent expression dynamics for both markers. Significance was determined using the Mann-Whitney U test.
    Immunofluorescence If Microscopy, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/immunofluorescence+microscopy/pmc12505278-210-10-16
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    ( A ) Left : Protein–protein interaction network centered on P4HA2, illustrating its context-dependent interactions with ER- and nucleoplasm-localized proteins. Right : Real P4HA2 images (red border) and ProtiCelli -generated images of ten interaction partners for two representative cells, grouped by interaction context (gold: ER/collagen; blue: mRNA processing). The rightmost column shows the binarized spatial interaction overlap between P4HA2 and the mean projection of proteins within partner groups. Scale bar, 10 µm. ( B ) Representative real and ProtiCelli -generated fluorescence microscopy images for four proteins (MECP2, BABAM2, YWHAB, BPTF) under Paclitaxel, untreated, and Vorinostat conditions, demonstrating the model’s ability to capture single-cell expression variance across treatment contexts. Scale bar, 10 µm. ( C ) Boxplots comparing ProtiCelli -generated versus real normalized protein intensities for ten representative proteins across treatment conditions. Median values are annotated; statistical significance (Mann-Whitney U test) is indicated by bracket annotations. ( D ) Scatter plots comparing average foreground intensity between real and ProtiCelli -generated images for twelve cell cycle-regulated proteins. Each point represents one single cell and the red line denotes the linear fit with 95% confidence interval. Pearson correlation coefficients ( r ) are reported per protein. ( E ) Representative real and ProtiCelli -generated immunofluorescence images of the FUCCI markers CDT1 (A-549) and GMNN (MDA-MB-467). Protein signal is shown in green; microtubule staining (red), derived from real images, is identical across all panels. Scale bar, 10 µm. ( F, G ) Generated versus measured FUCCI marker intensities of CDT1 and Geminin (GMNN) across cell cycle stages (G1, G1/S, G2) without fine-tuning, after fine-tuning, and for real images, in A-549 ( F ) and MDA-MB-468 ( G ) cell lines. Fine-tuned ProtiCelli accurately capture cell-cycle-dependent expression dynamics for both markers. Significance was determined using the Mann-Whitney U test.

    Journal: bioRxiv

    Article Title: Generative machine learning unlocks the first proteome-wide image of human cells

    doi: 10.64898/2026.03.31.715748

    Figure Lengend Snippet: ( A ) Left : Protein–protein interaction network centered on P4HA2, illustrating its context-dependent interactions with ER- and nucleoplasm-localized proteins. Right : Real P4HA2 images (red border) and ProtiCelli -generated images of ten interaction partners for two representative cells, grouped by interaction context (gold: ER/collagen; blue: mRNA processing). The rightmost column shows the binarized spatial interaction overlap between P4HA2 and the mean projection of proteins within partner groups. Scale bar, 10 µm. ( B ) Representative real and ProtiCelli -generated fluorescence microscopy images for four proteins (MECP2, BABAM2, YWHAB, BPTF) under Paclitaxel, untreated, and Vorinostat conditions, demonstrating the model’s ability to capture single-cell expression variance across treatment contexts. Scale bar, 10 µm. ( C ) Boxplots comparing ProtiCelli -generated versus real normalized protein intensities for ten representative proteins across treatment conditions. Median values are annotated; statistical significance (Mann-Whitney U test) is indicated by bracket annotations. ( D ) Scatter plots comparing average foreground intensity between real and ProtiCelli -generated images for twelve cell cycle-regulated proteins. Each point represents one single cell and the red line denotes the linear fit with 95% confidence interval. Pearson correlation coefficients ( r ) are reported per protein. ( E ) Representative real and ProtiCelli -generated immunofluorescence images of the FUCCI markers CDT1 (A-549) and GMNN (MDA-MB-467). Protein signal is shown in green; microtubule staining (red), derived from real images, is identical across all panels. Scale bar, 10 µm. ( F, G ) Generated versus measured FUCCI marker intensities of CDT1 and Geminin (GMNN) across cell cycle stages (G1, G1/S, G2) without fine-tuning, after fine-tuning, and for real images, in A-549 ( F ) and MDA-MB-468 ( G ) cell lines. Fine-tuned ProtiCelli accurately capture cell-cycle-dependent expression dynamics for both markers. Significance was determined using the Mann-Whitney U test.

    Article Snippet: Large-scale initiatives such as the Human Protein Atlas (HPA) have systematically mapped protein expression and subcellular localization across human cell types using immunofluorescence (IF) microscopy, imaging one protein at a time alongside reference markers that delineate cellular landmarks ( , ).

    Techniques: Generated, Fluorescence, Microscopy, Single Cell, Expressing, MANN-WHITNEY, Immunofluorescence, Staining, Derivative Assay, Marker